Part:BBa_K4209008

Composite part containing tal-fjo gene and SULT1A1 gene

This composite part is responsible for the production of Zosteric acid from tyrosine which is naturally present in cells. Tal-fjo is responsible for the first catalytic step, while SULT1A1 is responsible for the second. (Figure 1)

Figure 1. Schematic representation of the ZA production pathway The Tal-fjo enzyme converts the tyrosine, naturally present in the bacteria, into pHCA. CysP or CysPUWA are responsible for the uptake of sulphate into the cell. The CysDNC enzymes replace two phosphate groups of ATP by the sulphate. The sulphate carrier is used by SULT1A1 to incorporate sulphate into coumaric acid creating zosteric acid. CysQ molecule is essential to recycle ATP and allow for a second cycle to start.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2515
  Illegal PstI site found at 1380
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2515
  Illegal NheI site found at 2531
  Illegal PstI site found at 1380
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2515
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2515
  Illegal PstI site found at 1380
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2515
  Illegal PstI site found at 1380
  Illegal AgeI site found at 1002
  Illegal AgeI site found at 1344
  Illegal AgeI site found at 1434
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1879